Identification of any DNA sources, including accession numbers and addresses of depositories,
Procedure used to isolate and purify any gene(s),
Procedure used to synthesize and label any probe(s),
Procedure used to prepare any genomic or cDNA libraries,
Procedure used to enrich any DNA of interest,
Procedure and conditions of hybridization,
Procedure used for cloning,
Procedure used for characterizing clones,
Procedure used to construct vector(s),
Identification and sources of preferred and alternative cloning vectors, promoters, terminators, etcetera,
Preferred and other host cells for transformation, including accession numbers and addresses of depositories,
Preferred culture conditions,
Procedures for purification of protein(s),
Procedures for incorporating the protein into useful product(s), and
Nature and source of all trademarked material used in making the invention.
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